Pierce bca protein assay kit, thermo scientific vwr. Protocol for bca protein assay using a nanodrop 20002000c spectrophotometer subject. Bca standard protocol assay was performed on the modulus using the absorbance module and the 560 nm filter. The pierce micro bca protein assay kit is a threecomponent version of our bca reagents, optimized to measure total protein concentration of dilute protein solutions 0.
Dilution scheme for standard test tube protocol and microplate procedure working range 202,000gml vial volume of diluent. This is the protocol to be used in the determination of total protein in brain tissue by the pierce bca method. Pierce bca protein assay kit, thermo scientific assays cellular assays this bca protein assay kit is a twocomponent, highprecision, detergentcompatible assay reagent set to measure a562 nm total protein concentration compared to a protein standard. Bca protein assay reagent bicinchoninic acid youtube. Thermo scientific pierce protein assay technical handbook nugi. Bsa bovine serum albumin is used as the standard and samples are.
Cool the tubes to room temperature and transfer 1ml sample to a cuvette. Micro bca protein assay kit thermo fisher scientific. Pierce bca protein assay kit from thermo scientific. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric. This protocol makes use of thermo scientifics bca protein assay in a 96well tissue culture plate format to measure the amount of protein in small volumes of sample.
Tools and reagents for improved quantitation of total or specific proteins. Pierce bca protein assay kit thermo fisher scientific. Set a spectrophotometer to 562nm and blank with water. Measuring protein concentration using the bca protein. Similar bsa standard curves are obtained using the pierce rapid gold bca protein assay kit for 5 minutes at room temperature as compared to the traditional bca assay for 30 minutes at. Pierce bca protein assay kit university of arizona. An adaptation of the traditional bca assay, the rapid gold bca protein assay kit. Studies of the bca assay for determination of total.
Pierce microplate bca protein assay kit fisher scientific. Thermo scientific pierce protein assay technical handbook. Compare all available bca protein assays features of the micro bca protein assay kit include. Reducing agent compatible, sufficient reagents to assay 250 samples or standards of 25l each. The thermo scientific pierce bca protein assay combines the proteininduced biuret reaction with the highly sensitive and selective colorimetric detection of the resulting cuprous cation. Thermo scientific pierce microplate bca protein assay kit. Micro bca protein assay kit is a readytouse, detergentcompatible, western blot related, total protein analysis reagent, used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs.
The quantipro bca assay kit is based on the same principals as the bca1 kit, except the quantipro bca kit gives a linear response from 0. Pierce bca protein assay kit, sufficient reagents for 250 testtube or 2500 microplate assays. Bicinchoninic acid protein assay kit bca1 technical. Medical program director pierce county emergency medical services department of emergency management 2501 south 35th street, suite d tacoma wa 984097405 2537987722 a sincere thank you to all those individuals who attended the monthly meetings to write. Measuring protein concentration using the bca protein assay with. This special adaptation of the popular pierce bca protein assay method enables accurate protein concentration measurement for samples containing dtt, 2me or tcep. Available in tube and microplate formats, these reducing agent compatible rac bca kits extend the already broad reagent compatibility of the bca protein assay protocol to include. The glomax discover system and pierce bca protein assay provide a simple method for. Pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible protein assay for determination of protein concentration. Thermo scientific pierce micro bca protein assay kit standard protocol. Tools and reagents for improved quantitation of total or.
Bicinchoninic acid protein assay kit catalog numbers bca1 and b9643 technical bulletin synonym. The micro bca protein assay kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature 60c, test tube procedure only. Thermo scientific pierce bca protein assay kit reducing agent compatible. The bca protein assay is a popular method for colorimetric detection and quantitation of total protein. We use the pierce micro bca kit to determine protein concentrations in solutions that contain. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total. Thermo scientific pierce bca protein assay reagent b.
The pierce bca assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications. Studies of the bca assay for determination of total protein in allergen extracts title swedish abstract the aim of this thesis has been to evaluate and to optimize the bca protein assay for quantification of total protein. Used in more labs than any other detergentcompatible protein assay, pierce bca reagents provide accurate determination of protein concentration with most sample types. As different cell types have different levels of ldh activity, the cell density for the assay will depend on the cell type used and will need to be optimized by the enduser to obtain accurate experimental results a. The pierce bca protein assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications. The protocol has been optimized for use in a microplate to reduce the required sample volume. Pierce microplate bca protein assay kit reducing agent compatible, sufficient reagents for. Bca protein assay kit ab102536 provides a simple, rapid, detergent tolerant up to 5% procedure for determining the concentration of proteins in solution. Bca benefitcost analysis a method for determining the potential positive effects of a mitigation measure and comparing them to the cost of the measure. The pierce rapid gold bca protein assay kit is a fast, twocomponent, highprecision, detergentcompatible assay optimized to determine total protein concentration. Abcam s bca protein assay kit reducing agent compatible microplate ab207003 measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Bca product description protein determination is one of the most common operations performed in biochemical research. Standard 2 ml assay protocol this is the standard assay that can be performed in a test tube. Dc protein assay instruction manual for technical service call your local biorad office or in the u. The micro bicinchoninic acid bca protein assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and is a modification of the bicinchoninic acid bca protein assay for dilute protein samples 0. With the fema bca modules, the positive effect is a reduction in future damages from natural hazards. Streamline bcabased protein quantitation molecular devices. The kits include bovine serum albumin, which is used to set up a standard curve from which the. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein.
An adaptation of pierces bca protein assay reagent kit product no. The principle of lowry assay, biuret assay, and bicinconinich bca assay protein assays duration. The principle of the bicinchoninicacid bca assay is similar to the lowry procedure,1 in that both rely on the. Pierce bca bicinchoninic acid reagents provide accurate determination of protein concentration with most sample types encountered in protein research. Protocol for bca protein assay using a nanodrop 8000 spectrophotometer subject. The pierce micro bca protein assay reagent kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. Thermo scientific pierce bcaeiwitassay bca protein. The thermo scientific pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Thermo scientific pierce bca protein assay kit remains one of the most popular protein quantitation methods worldwide.
The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Biomedical and biological sciences 59,406 views 24. The bca protein assay kit from thermo scientific pierce product no. It uses the same copperchelating technology as the wellknown traditional pierce bca protein assay and provides comparable accuracy bu. Bca protein assay kit from thermo scientific pierce. Micro bca protein assay kit pierce micro bicinchoninic. Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater proteintoprotein uniformity. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional differences, providing greater proteintoprotein uniformity. Pierce bca reagents provide accurate determination of protein concentration with most sample types encountered in protein research. I have a technical question regarding the bca assay. The thermo scientific pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible assay reagent set to measure a562nm total protein concentration compared to a protein standard. If they can be stored and reused to create another standard curve, what is recommended for storage temp. This was done by analyzing the precision, accuracy, linearity and range of the method.
This method combines the well known reduction of cu. Protocol for bca protein assay using a nanodrop 8000. The bicinchoninic acid assay bca, also referred to as the smiths assay, is a biochemical assay designed by paul smith in 1985. The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format. Dilution scheme for enhanced test tube protocol working range 5250gml. Bca protein assay kit from pierce provides a simple method for quantifying protein over a large range. The thermo scientific pierce bca protein assay kit is a detergent compatible formulation based on bicinchoninic acid bca for the.
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